The study investigates the role of Smad7 overexpression in intestinal epithelial cells. Mice with epithelial-specific Smad7 overexpression developed spontaneous ileal inflammation characterized by epithelial damage, mucus depletion, increased permeability, and CD8 positive T cell infiltration. Altered purine metabolism with reduced CD73 expression led to decreased adenosine levels, and adenosine supplementation restored epithelial function and reduced inflammation.
Smad7 overexpression in epithelial cells induces ileal inflammation through impaired purine metabolism and reduced adenosine production. Restoration of adenosine rescues epithelial barrier function and reduces inflammation.
Smad7 overexpression conditional Knockin mouse in intestinal epithelium Smad7TgCre+ model
Inflammatory bowel disease, Crohn disease, mucosal immunity, epithelial barrier dysfunction, purine metabolism
Conditional epithelial overexpression of Smad7 using Cre system, spatial transcriptomics, metabolomics analysis, cytokine profiling, histopathology, adenosine rescue experiments
From model design to experimental results
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