This study presents the development and characterization of novel single-domain antibodies (nanobodies) that specifically recognize human CD4. After thorough in vitro analysis, selected nanobodies were converted into immune tracers for noninvasive molecular imaging. Using a human CD4 Knockin mouse model, the study demonstrated that radiolabeled nanobodies could specifically accumulate in CD4⁺ T cell-rich tissues, enabling high-resolution imaging via immunoPET/MR.
Radiolabeled CD4-specific nanobodies selectively accumulated in CD4⁺ T cell-rich organs in the hCD4-KI mice, demonstrating their potential as noninvasive imaging agents for monitoring CD4⁺ T cells in vivo.
Human CD4 Knockin (hCD4-KI) mouse model, engineered to express human CD4 in place of the murine counterpart, allowing for in vivo evaluation of human CD4-targeting agents.
Immunotherapy, CD4⁺ T cell imaging, Nanobody-based diagnostics, Molecular imaging, PET/MR
Humanized Knockin model, CD4 gene replacement, Nanobody development, ImmunoPET imaging
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
