The authors generated a reporter mouse carrying a knock‑in eGFP‑TRF1 fusion allele to study TRF1 in stem cell compartments. eGFP‑TRF1 expression was maximal in adult stem cell niches. During iPSC reprogramming, eGFP‑TRF1 was highly expressed and selecting for TRF1‑high cells correlated with higher pluripotency (teratoma and chimera formation). TRF1 is required for both induction and maintenance of pluripotency.
Reporter marks adult stem cell compartments; TRF1‑high iPS cells show higher pluripotency; TRF1 required for iPSC generation and maintenance
eGFP‑TRF1 Knockin reporter mouse
Stem cell biology, pluripotency, iPSC reprogramming, telomere biology, adult stem cell compartments
Knockin fusion of eGFP at TRF1 locus, reporter allele generation, iPSC derivation assays, teratoma assays, chimera formation tests
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