Axenis Immunodeficient Mouse Strain Portfolio
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Genetically Modified Immunodeficient Mouse Models
Our Axenis immunodeficient mouse model portfolio is based on the proprietary state-of-the-art BRGS™ mouse model and consists of the following strains:
- BRGS (BALB/c Rag2tm1Fwa Il2rgtm1Cgn SirpaNOD)
- BRGSF (BALB/c Rag2tm1Fwa Il2rgtm1Cgn SirpaNOD Flt3tm1lrl)
- BRGSF HLA-A2tg (genetically humanized for HLA-A2HHD–Tg(HLA-A/H2-D/B2M)1Bpe)
- BRGSF HLA-DR2tg (genetically humanized for HLA-DR2–Tg(HLA-DR2)Lfug)
- BRGSF HLA-A2tg HLA-DR2tg (double-humanized for HLA-A2HHD/DR2)
Reconstituted, Humanized Axenis Mice–Specific Tools for Human Biomedical Research
Axenis immunodeficient humanized mouse models offer new opportunities to address human-specific questions in a more relevant manner, facilitating translational research in several biomedical disciplines and approaches.
Axenis BRGS/BRGSF mice are excellent recipients for human hematopoietic cells. We routinely generate BRGS/BRGSF mice humanized for the immune system (HIS), using human CD34+ hematopoietic progenitor cells.
The Axenis BRGS/BRGSF-HIS mice contain all major human hematopoietic cell subsets, such as B cells, T cells (including CD4+ Treg cells), NK cells, and the myeloid compartment including dendritic cells (DCs), plasmacytoid cells (pDCs) and monocytes/macrophages.
Optimized formats of the Axenis BRGS/BRGSF-HIS mice are:
- BRGSF-DC – boosted for human dendritic cell content
- BRGS/BRGSF-ImStim – for robust immune responses
- BRGS/BRGSF-NK – boosted for human NK cell content
The human myeloid compartment can be boosted with a simple exogenous Flt3-ligand treatment in BRGSF mice that lack the corresponding mouse receptor (Flk2/Flt3). The boost of human dendritic cell accumulation potentiates human T cell function.
Strategy for HIS mouse generation
Human cell-boosting options in Axenis BRGS/BRGSF-HIS mice
Applications of Axenis BRGS/BRGSF-HIS mice
BRGS/BRGSF mouse models are in use for studies in fields such as:
Studies can span from the earliest R&D to preclinical safety/efficacy development stages. Performing screening on components of the human immune system in an in vivo context is likely to yield results that will more reliably predict human responses, as compared to those obtained using “standard” rodent models.
We design custom-made models that meet the specific needs of our customers. Exploratory work, as well as late preclinical studies, are performed in areas of activity such as:
- Hematopoietic efficacy/safety profiling of drugs and biologicals: covers molecules such as vaccines, adjuvants, biosimilars, small synthetic molecules, monoclonal antibodies, new formulations – in a variety of application fields
- Infectious diseases: humanized mice can support infection by a variety of human-tropic pathogens (e.g. HIV, CMV, RSV, EBV, Dengue virus, HTLV, etc.) and host–pathogen interactions, as well as new therapeutics, can be evaluated directly in BRGS-HIS mice
- Oncology: BRGS mice are particularly permissive to human tumor xenograft transplantation, and host–tumor interactions, as well as evaluation of therapeutics that require the presence of both a tumor and human immune cells can be evaluated in BRGS-HIS mice
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