This study evaluated human mast cell (hMC) development, distribution, and activation in CD34⁺ HSC-engrafted humanized mouse models. BRGSF mice supported hMC presence in skin, lung, and gut. hMCs expressed key markers including KIT, FcεRI, and MRGPRX2. Functional activity was confirmed via passive cutaneous anaphylaxis assay. Single-cell transcriptomics showed phenotypic diversity of engrafted hMCs.
BRGSF mice supported engraftment of functionally mature human mast cells in multiple tissues. hMCs showed appropriate surface marker expression and transcriptional heterogeneity, enabling in vivo study of mast cell biology and allergic responses.
Immunodeficient humanized mouse strain (BRGSF) engrafted with human CD34⁺ hematopoietic stem cells
Human mast cell biology, allergy modeling, in vivo humanized immune models, passive anaphylaxis assays, mast cell engraftment
Humanized mouse engraftment of CD34⁺ progenitors, flow cytometry of hCD45⁺ populations, immunofluorescence tissue imaging, single-cell RNA sequencing (skin, gut), passive cutaneous anaphylaxis assay, serum tryptase quantification
From model design to experimental results
Featured in 600+ scientific articles
Collaboration with 17 Top Pharmas,
170+ Biotechs and 380+ Academic Institutions
Generated with biopharma partners and in-house
and guaranteed freedom to operate
Models with certified health status from professional breeders in US and Europe
