Double Humanized Serum Albumin/Neonatal Fc Receptor Mouse Model

HSA/hFcRn

Our double humanized serum albumin/neonatal Fc receptor mouse model (HSA/hFcRn) maintains an autologous receptor-ligand interaction and mimics the physiological drug clearance in humans.

Applications

  • Accurate and predictive pharmacokinetic and pharmacodynamic studies of albumin-linked drugs
  • Development of conventional drugs and biologics with enhanced half-life through interaction with HSA/hFcRn
 

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HSA/hFcRn features

  • HSA and hFcRn are controlled by their respective endogenous mouse promoters, therefore displaying a physiological expression and regulation
  • Physiological expression level of HSA (within the normal range of 1.5–6 g/dL)
  • Normal blood chemistry
  • Murine SA and FcRn expression is abolished

HSA/hFcRn validation

The model has been co-validated by Albumedix, the biopharmaceutical operations spin out of Novozymes.

HSA/hFcRn model validation 1

Expression of hFcRn is physiological

Upper panel: hFcRn expression (qPCR) in duodenum (Du), jejunum (Je), ileum (iLe), large intestine (Li), liver (H), kidney (K), and lung (Lu) Lower panel: Immunohistochemical staining for hFcRn in HSA /FcRn humanized (large image) and wild-type (small insert) mice. No cross reactivity to mouse FcRn was seen in the wild-type mice (small insert).

HSA/hFcRn ICP model validation 2

Half-life of drug conjugated to human albumin is extended in our double humanized model

Albumin-bound drug 1: low affinity for hFcRn
Albumin-bound drug 2: high affinity for hFcRn

* For more validation data please contact us.

Reference

Viuff D, Antunes F, Evans L, Cameron J, Dyrnesli H, Thue Ravn B, Stougaard M, Thiam K, Andersen B, Kjærulff S, Howard KA. 2016. Generation of a double transgenic humanized neonatal Fc receptor (FcRn)/albumin mouse to study the pharmacokinetics of albumin-linked drugs. J Control Release. [PubMed]

Ready to be shipped to your lab

  • Cohorts available upon request
  • Studies can be carried out at your site or at your favorite CRO
  • VAF Elite/SOPF certification and worldwide delivery by professional breeders
  • Models provided with FTO on patent-protected technologies used for model generation