Immunodeficient SDRG Rat Model

Rat Model Catalog

Immunodeficient rats represent powerful tools to investigate the pathobiology of B cell disorders such as leukemias and lymphomas, as well as lymphoid compartment development.

They are valid alternatives to immunodeficient mice since, e.g., the liquid nature of malignancies like B cell lymphoma make downstream analysis quite challenging due to small blood volumes of mice.

Compared to the traditional immunodeficient nude rats, which lack T cells but maintain a normal repertoire of all other immune cells, including B and NK cells, the SDRG model is a double Knockout for Rag1 and IL-2Rγ genes (Rag1^-/- Il2rγ^-/-), resulting in a B, T and NK cell deficiency.

^{Request a quote here to get pricing, offers and SDRG rat model information by phone or email.}

Applications

The SDRG rat represents a valid preclinical model to study:

Lymphoid compartment development
B cell malignancies (e.g., leukemias, lymphomas)
Xenografts
Inflammatory disorders
Infectious diseases

Model features

Sprague Dawley genetic background
Lack of Rag1 and IL-2Rγ protein expression

SDRG Rat

Model validation

SDRG rats display no T, B and NK and NKT cells

SDRG rat model validation

Hemocytometer and flow cytometry count of T, B, NK, NKT and myeloid cells in SDRG and wild-type Sprague Dawley rats. SDRG rats display a significantly reduced number of T and B cells (upper graphics) and NK cells (lower graphics) compared to wild-type Sprague Dawley rats. Flow cytometry analyses of T, B, NK, NKT and myeloid cells (CD3⁺ TCRαβ⁺, CD3^- CD45R⁺, CD3^- TCRαβ^- CD335⁺ CD161⁺, CD3⁺ TCRαβ^- CD335⁺ CD161⁺, CD3^- CD45R^- CD161^- respectively) in blood and spleen.

* For more validation data please contact us.

Immunodeficient SDRG Rat Model

Applications

Model features

Model validation

Further reading

Ready to be shipped to your lab