Immunodeficient SDRG Rat Model

Catalog Rat Models

Immunodeficient rats represent powerful tools to investigate the pathobiology of B cell disorders such as leukemias and lymphomas, as well as lymphoid compartment development.

They are valid alternatives to immunodeficient mice since, e.g., the liquid nature of malignancies like B cell lymphoma make downstream analysis quite challenging due to small blood volumes of mice.

Compared to the traditional immunodeficient nude rats, which lack T cells but maintain a normal repertoire of all other immune cells, including B and NK cells, the SDRG model is a double Knockout for Rag1 and IL-2Rγ genes (Rag1-/- Il2rγ-/-), resulting in a B, T and NK cell deficiency.

 

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Applications

The SDRG rat represents a valid preclinical model to study:

  • Lymphoid compartment development
  • B cell malignancies (e.g., leukemias, lymphomas)
  • Xenografts
  • Inflammatory disorders
  • Infectious diseases

Model features

  • Spraque Dawley genetic background
  • Lack of Rag1 protein expression
  • Lack of IL-2Rγ protein expression

SDRG Rat

 

Model validation

SDRG rat model validation

SDRG rats display less than 2% of T cells and less than 0.5% of NK and B cells compared to wild-type Sprague Dawley rats.

Hemocytometer and flow cytometry count of B, T and NK cells in SDRG and wild-type Sprague Dawley rats. SDRG rats display a significantly reduced number of T and NK cells (A) and peripheral B cells (B) compared to wild-type Sprague Dawley rats. C) Flow cytometry analyses of T, B and NK cells showing that mature T, B and NK cell populations (TCR α/β+, CD45R+ IgM+, and CD161+, respectively) are significantly reduced in SDRG rats.

* For more validation data please contact us.

Ready to be shipped to your lab

  • Cohorts available upon request
  • VAF/SPF certification and worldwide delivery by professional breeders
  • Models provided with FTO on patent-protected technologies used for model generation