An inducible Knockdown cell line defines a model in which a specific target of interest can be shut down upon treatment.

We use SMASh technology to generate drug inducible protein degradation models. Through a single genetic modification, the addition of a self-cleaving degron tag to a protein of interest allows its inducible, and reversible, degradation upon treatment (further reading).

genOway has an exclusive license for the use of the patented SMASh technology. (See press release)

Strengths of inducible Knockdown cell lines

  • Inducible and reversible system
  • One cell line = several models with different level of expression of the protein of interest
  • Reliable: each cell line is matched with an isogenic control cell clone
  • Feasible in all genetic backgrounds and cell types

Limitations of inducible Knockdown cell lines

  • Efficiency of down regulation depends on the target and cell line of interest
  • Genetic modification of the gene of interest may deregulate neighboring genes, inducing a non-specific phenotype
  • Challenging in cells with high polyploidy

→  Risks can be strongly minimized by applying in-depth bio-informatic, genetic, and bibliographic analyses
→ An alternative model is a functional Knockout by introducing a
point mutation to produce an inactive protein

Proof of concept

Immune checkpoint PD-L1 has been identified as a therapeutic target in immuno-oncology and different treatments have been successfully developed to target this protein in oncology.

We developed mouse tumor MC38 cells invalidated for mouse Pd-l1 and overexpressing a SMASh-tagged form of human PD-L1 to reversibly shut down its expression in these cells, thus obtaining cells entirely devoid of PD-L1 upon drug treatment.

Two cell lines developed:

  • MC38-SMASh-hPD-L1, with the tag inserted in N-term of the targeted protein
  • MC38-hPD-L1-SMASh, with the tag inserted in C-term of the targeted protein


For academic research:

  • Determine and study main functions of the gene and/or protein
  • Loss-of-gene-function studies
  • In vitro recapitulation of a human disease

For bio-pharmaceutical research & development:

  • In vitro target validation
  • Mimic human disease for drug studies and screenings
  • Positive control for antagonist drug screening
  • In vitro model for targeted protein degradation


Related ressources et publication
No results
No results
No items found.
No results
No results
No results
No results

Discover related products to

Inducible Knockdown cells

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.

Reporter KI cells

A reporter Knockin cell line is a useful model when there are no available antibodies for the target or when protein measurement is laborious and costly.

Point mutation

Point mutation KI cells

A point mutation Knockin cell line has one or more mutated nucleotides that alter the protein amino acid sequence, changing its functions or properties.

Permissive locus

Quick KI cells

A quick Knockin cell line has a transgene inserted into permissive loci (e.g., Rosa26). The transgene overexpression is constitutively or inducibly.


Constitutive KO cells

A constitutive Knockout cell line refers to a conventional KO; a model in which the target gene is permanently inactivated, and no protein is produced.

Discover our off-the-shelf models.

Learn more

Inducible Knockdown cell lines

Get in touch about

Let us know how we can help

In order to provide you the content requested, we need to store and process your personal data. If you consent to us storing your personal data for this purpose, please tick the checkbox below.

From time to time, we would like to contact you about our products and services, as well as other content that may be of interest to you. If you consent to us contacting you for this purpose, please tick below.

You can unsubscribe from these communications at any time. For more information on how to unsubscribe, our privacy practices, and how we are committed to protecting and respecting your privacy, please review our Privacy Policy.

Thank you! Your submission has been received!
Oops! Something went wrong while submitting the form.