NK cell recruitment into A549 TME in genO-BRGSF-HIS mice

Background

Natural killer (NK) cells are key components of the innate system, and their high cytotoxicity has been associated to a more favorable prognosis of cancer. Therefore, strategies focusing on enhancing the effector function of NK cells are being investigated. Assessment of NK-targeting therapies in humanized mice is often challenging, as human NK cell levels are low, requiring supplementation with human IL-15 to enhance their development. Here we describe the recruitment of NK cells in the tumor microenvironment (TME) of A549 lung cancer tumor model in genO-BRGSF-HIS mice (BALB/c Rag2^-/-, IL2Rγ^-/-, SIRPα^NOD and Flt3^-/- immunodeficient mice reconstituted with human cord blood CD34⁺ cells), without the need of supplementation with human IL-15.