The study presents a novel immunocompetent mouse model with a strategically humanized CD3 epitope that enables in vivo evaluation of human CD3-targeting T-cell engagers. The model preserves normal T-cell development and supports effective testing of bispecific T-cell engagers, showing specific T-cell-mediated depletion of B cells in vivo.
Maintains full immune competence while enabling preclinical testing of anti-human CD3 T-cell engagers; demonstrates potent B cell depletion with CD3/CD20 bispecific antibody.
Humanized CD3ε epitope Knockin (hCD3E-epi) mouse model generated by genOway, with a human epitope sequence replacing the murine N-terminal CD3ε region, enabling recognition by clinical anti-hCD3 antibodies.
Immuno-oncology, T-cell engagers, CD3-targeting bispecific antibodies, Preclinical immunotherapy
Humanized Knockin model, CD3ε epitope substitution, Immunocompetent background, T-cell engager evaluation
From model design to experimental results
Tailor-made solutions adapted to scientific questions
Comprehensive dataset package
Generated with biopharma partners and in-house
Scientific follow-up and advice along the project
Collaborative approach for problem solving and development of innovative models
Breeding facilities in US and Europe
Certified health status from professional breeders
