Quick Knockin Cell Lines (Neutral Loci Targeting: Rosa26, Hprt, AAVS1)

 



Get supplemental information, a quote, and estimated timeframe for generating your safe harbor Knockin cell line.

 

A Quick Knockin™ cell line defines a model in which a transgene is inserted in a permissive locus, also known as neutral locus or safe harbor, such as example Rosa26, Hprt and AAVS1. The transgene can be constitutively or inducibly overexpressed.

Targeting those loci enable a full control of the copy number and avoid transgene silencing or deregulation of neighboring genes.

Infographic: Permissive locus Knockin cell model

 

Gene of interest can be:

⇒ Constitutively expressed

⇒ Conditionally expressed by using site-specific recombination (see: Cre-ERt2 or FLEx)

 

 

Typical applications for permissive locus Knockin cell models


For academic research:
  • Gene function studies
  • Study disease-causing transgene, mutated or not
For bio-pharmaceutical research & development:
  • Compound screening
  • Selectivity screening
  • Drug affinity testing
  • Compound profiling

Strengths and limitations of permissive locus Knockin cell models

+
  • Stable and predictable expression pattern
  • Almost no position effect variegation
  • Almost no transgene silencing
  • No deregulation of neighboring genes
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  • 1. Exogenous regulation may result in lower physiological relevancy
    2. Challenging when multiple isoforms have been described
    3. Problem of stoichiometry if interacting with other partners for signaling
    → Must be solved through an in-depth scientific analysis and a rationale risk assessment prior model development
  • Not functional for expression of non-dominant mutations
    → Choose alternative approaches such as example point mutation Knockin

 

Follow this link if you seek another customized cell line model.