Quick Knockin Cell Lines (Neutral Loci Targeting: Rosa26, Hprt, AAVS1)
A Quick Knockin™ cell line defines a model in which a transgene is inserted in a permissive locus, also known as neutral locus or safe harbor, such as example Rosa26, Hprt and AAVS1. The transgene can be constitutively or inducibly overexpressed.
Targeting those loci enable a full control of the copy number and avoid transgene silencing or deregulation of neighboring genes.
Gene of interest can be:
⇒ Constitutively expressed
Typical applications for permissive locus Knockin cell models
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of permissive locus Knockin cell models
- Stable and predictable expression pattern
- Almost no position effect variegation
- Almost no transgene silencing
- No deregulation of neighboring genes
- 1. Exogenous regulation may result in lower physiological relevancy
2. Challenging when multiple isoforms have been described
3. Problem of stoichiometry if interacting with other partners for signaling
→ Must be solved through an in-depth scientific analysis and a rationale risk assessment prior model development
- Not functional for expression of non-dominant mutations
→ Choose alternative approaches such as example point mutation Knockin
Follow this link if you seek another customized cell line model.