Quick Knockin Cell Lines (Neutral Loci Targeting such as Rosa26)
A Quick Knockin™ cell line defines a model in which a transgene is inserted in a permissive locus, also known as neutral locus or safe harbor, such as Rosa26. The transgene can be constitutively or inducibly overexpressed.
Targeting those loci enables full control of the copy number and avoids transgene silencing or deregulation of neighboring genes.
Typical applications for Quick Knockin cell models
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of Quick Knockin cell models
- Stable and predictable expression
- Almost no position effect variegation
- Almost no transgene silencing
- No deregulation of neighboring genes
- 1. Exogenous regulation may result in lower physiological relevancy
2. Limited physiological relevancy when multiple isoforms have been described
3. Problem of stoichiometry if interaction with other partners for signaling
→ Must be solved by an in-depth scientific analysis and a rationale risk assessment prior to model development, all included in our project feasibility assessment
- Not functional for expression of non-dominant mutations
→ Choose alternative approaches such as, e.g., point mutation Knockin
Follow this link if you seek another customized cell line model.