Point Mutation Knockin Cell Lines

 



Get supplemental information, a quote, and estimated timeframe for generating your modified cell line.

 

A point mutation Knockin cell line defines a model in which one or more nucleotides are constitutively mutated.

The insertion, deletion, nonsense and sense mutations alter the amino acid sequence of a given protein, and so changes its functions or properties.

Infographic: Point mutation Knockin cell model

Typical applications for point mutation Knockin cell lines


For academic research:
  • Decipher protein function (gain or loss of function)
  • Investigate disease-causing mutations
For bio-pharmaceutical research & development:
  • Pharmacological off-target and efficacy studies by suppressing xenobiotics or antibody binding
  • Study drug resistant mutants
  • Synthetic mortality screening in cancers

Strengths and limitations of point mutation Knockin cell lines

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  • High physiological relevancy of the scientific data obtained from the model (regulatory elements conserved, under control of endogenous promoter, expression of all splice variants, etc.) = cleaner way than classical KO where the protein is absent
  • Reliable since each cell line is matched with an isogenic control cell clone
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  • Unpredicted alteration of splicing or 3D-protein structure

    →  If the genetic modification creates a dominant mutation, an overexpression of the point mutant in a safe harbor site (Quick Knockin™), might be a valuable option

 

Follow this link if you seek another customized cell line model.