Point Mutation Knockin Cell Lines
A point mutation Knockin cell line defines a model in which one or more nucleotides are constitutively mutated.
The insertion, deletion, nonsense and sense mutations alter the amino acid sequence of a given protein, and so changes its functions or properties.
Typical applications for point mutation Knockin cell lines
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of point mutation Knockin cell lines
- High physiological relevancy of the scientific data obtained from the model (regulatory elements conserved, under control of endogenous promoter, expression of all splice variants, etc.) = cleaner way than classical KO where the protein is absent
- Reliable since each cell line is matched with an isogenic control cell clone
- Unpredicted alteration of splicing or 3D-protein structure
→ If the genetic modification creates a dominant mutation, an overexpression of the point mutant in a safe harbor site (Quick Knockin™), might be a valuable option
Follow this link if you seek another customized cell line model.