Reporter Knockin Cell Lines
A reporter Knockin cell line defines a model in which fluorescent, bioluminescent proteins or biochemical tags are inserted into the genome. The reporter can replace a gene, be fused to a protein or co-express with the target gene.
It is a particularly useful model when there are no available antibodies for the target protein or when protein measurement is laborious and costly.
Typical applications for reporter Knockin cell models
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of reporter Knockin cell models
- Simple and cost-effective monitoring tool of desired cellular events without using antibodies
- Numerous technical options optimized for different scientific questions: regulation, expression, quantififcation, etc.
- Fusing the reporter to a protein may alter protein conformation, localization and functionality
→ Limitation can be bypassed by applying IRES co-expression technology
- 3' UTR approaches provide only relative quantification: potential dysregulation of expression level and challenging when multiple isoforms exist
→ Must be solved by an in-depth scientific analysis and a rationale risk assessment prior to model development, all included in our project feasibility assessment
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