Related resources and publications
VISTA - safety and PK profiling
Nat Commun
The hVISTA mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint VISTA in fully immunocompetent mice.
The humanized VISTA mouse model (hVISTA) is developed by Knockin at the mouse VISTA locus, and expresses a chimeric VISTA with a human extracellular, human transmembrane and murine intracellular domain.
The design also gives access to VISTA conditional Knockout mice for a tissue-specific assessment of compounds’ specificity and toxicity.
hVISTA and mVISTA expression on freshly isolated splenocytes on A) Tregs, B) CD11bHigh, C) conventional CD4 (CD4conv), and D) CD8 T cells. VISTA is not detected on cells from VISTA Knockout homozygous mice.
Mice of indicated genotype were inoculated with MC38 tumor cells s.c. and treated αKLH (control), αmPD-1, αhVISTA or αmPD-1 + αhVISTA (combination). Tumor volume measurement in A) wild-type and B) hVISTA Knockin (KI) homozygous mice at different time points. Results are expressed as mean±SEM. C) Survival of treated hVISTA KI homozygous mice analyzed up to 50 days post MC38 tumor inoculation.
VISTA - safety and PK profiling
Nat Commun
The hGITR/Foxp3 mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint GITR. The red fluorescent protein regulated under the Foxp3 promoter allows you to efficiently monitor and sort Foxp3-expressing cells from different lymphocyte lineages and lymphoid organs.
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