Knockin Cell Lines

 



Get supplemental information, a quote, and estimated timeframe for generating your Knockin cell line.

 

A Knockin cell line defines a cell line in which an exogenous gene is inserted in a native locus of interest.

The inserted gene or gene sequence expression is regulated under the native promoter, which provides a physiologically relevant and secure expression.

 

Infographic: Conventional Knockin cell model

Typical applications for conventional Knockin cell lines


For academic research:
  • Study gene expression as part of specific functions of signaling pathways
  • Study expression of mutated isoforms
For bio-pharmaceutical research & development:
  • Expression of targets for compound screening
  • Selectivity screening
  • Drug affinity testing
  • Compound profiling
  • Bioproduction
  • Comparison of target responses to xenobiotics

Strengths and limitations of conventional Knockin cell lines

+
  • Preservation of endogenous level of gene expression (native promoter)
  • Reliable gene expression level
-
  • Ablation of endogenous gene controlled by promoter of interest
    → Can be avoided by using IRES
  • Level of expression might be too weak
    → Can be compensated by the use of a WPRE (Woodchuck hepatitis virus Post-transcriptional Regulatory Element)

 

Follow this link if you seek another customized cell line model.