Cell line generation

Predictive cell models to elevate the precision and efficiency of your research.

Genetically engineered cell lines

Our CRISPR/Cas9 gene-editing platform is optimized for customized cell line development .

A major benefit for you and your research is our rigorous procedure to quickly assess the feasibility of using your own cell line, and adapt our process to your cell line's specifics, if need be. Genetic modifications designed and performed with our standards in your favorite cell line optimize the use of your existing data and avoid the need for new standardization experiments.

Our state-of-the-art knowledge of the technologies we use, combined with thorough design assessment and optimized proprietary protocols, allow us to ensure reliable and reproducible performance in a wide range of rodent or human cell lines, including tumor cell lines for syngeneic models and hard-to-edit suspension cells:

  • Breast cancer, colorectal cancer, melanoma, insulinoma, and glioblastoma cell lines such as MC38, CT26, 4T1, B16F10, GL261 mouse lines
  • B-cell lymphoma, macrophage, and leukemia cell lines, including HBL1 and VL51 human cell lines
  • Embryonic stem cells, induced pluripotent stem cells, neural stem cells, and more

Along with its expertise, we provide our customers with FTO, the freedom to operate on key technologies, including CRISPR/Cas9.

Our services for

Cell line generation

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Knockout
Customized
Cells

Inducible Knockdown cells

Through a single genetic modification, the addition of a self-cleaving degron tag to a protein of interest allows its inducible, and reversible, degradation upon treatment.

Knockin
Reporter
Customized
Cells

Reporter KI cells

A reporter Knockin cell line is a useful model when there are no available antibodies for the target or when protein measurement is laborious and costly.

Point mutation
Customized
Knockin
Cells

Point mutation KI cells

A point mutation Knockin cell line has one or more mutated nucleotides that alter the protein amino acid sequence, changing its functions or properties.

Knockin
Permissive locus
Customized
Cells

Quick KI cells

A quick Knockin cell line has a transgene inserted into permissive loci (e.g., Rosa26). The transgene overexpression is constitutively or inducibly.

Knockout
Constitutive
Customized
Cells

Constitutive KO cells

A constitutive Knockout cell line refers to a conventional KO; a model in which the target gene is permanently inactivated, and no protein is produced.

Related ressources et publication
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