Knockin Cell Lines
A Knockin cell line defines a cell line in which an exogenous gene is inserted in a native locus of interest.
The inserted gene or gene sequence expression is regulated under the native promoter, which provides a physiologically relevant and secure expression.
Typical applications for conventional Knockin cell lines
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of conventional Knockin cell lines
- Preservation of endogenous level of gene expression (native promoter)
- Reliable gene expression level
- Ablation of endogenous gene controlled by promoter of interest
→ Can be avoided by using IRES
- Level of expression might be too weak
→ Can be compensated by the use of a WPRE (Woodchuck hepatitis virus Post-transcriptional Regulatory Element)
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