Genetically Engineered Cell Lines
Our CRISPR/Cas9 gene-editing platform is optimized for customized cell line development .
A major benefit for you and your research is our rigorous procedure to quickly assess the feasibility of using your own cell line, and adapt our process to your cell line's specifics, if need be. Genetic modifications designed and performed with our standards in your favorite cell line optimize the use of your existing data and avoid the need for new standardization experiments.
Our state-of-the-art knowledge of the technologies we use, combined with thorough design assessment and optimized proprietary protocols, allow us to ensure reliable and reproducible performance in a wide range of rodent or human cell lines, including tumor cell lines for syngeneic models and hard-to-edit suspension cells:
- Breast cancer, colorectal cancer, melanoma, insulinoma, and glioblastoma cell lines such as MC38, CT26, 4T1, B16F10, GL261 mouse lines
- B-cell lymphoma, macrophage, and leukemia cell lines, including HBL1 and VL51 human cell lines
- Embryonic stem cells, induced pluripotent stem cells, neural stem cells, and more
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genOway has developed a MC38-hPD‑L1 cell line, expressing high levels of human PD-L1 and Knockout for mouse Pd-l1, with demonstrated capacity of forming tumors in vivo. Other cellular models are under development. |
Along with its expertise, genOway provides its customers with FTO, the freedom to operate on a broad range of key technologies, including CRISPR/Cas9 technology.
Our cell line services
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Constitutive Knockouts Permanent inactivation of the target gene, and total absence of the protein |
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Inducible Knockdowns Inducible, and reversible, target protein degradation upon treatment |
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Point mutation Knockins Constitutively mutated nucleotides affecting the function of a given protein (gain/loss of function) |
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Reporter Knockins Insertion of fluorescent, bioluminescent proteins or biochemical tags |
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Quick Knockins Controlled gene expression by targeting permissive loci such as Rosa26 |
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"Simple" Knockins Add a gene sequence of interest to an endogenous locus |
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Learn more about CRISPR efficiency for large Knockin in our last R&D paper published in Scientific Reports.
Read our commentary on how induced pluripotent stem cells revolutionized regenerative biology.
Learn more about our cell line engineering service:
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Follow this link if you are looking for another transgenic model.