Preclinical Double-Humanized PD-1/VISTA Mouse Model

Design of the hPD‑1/hVISTA mouse

The double-humanized PD-1 and VISTA mouse model (hPD-1/hVISTA) was generated by intercrossing hPD-1 and hVISTA mice.

hPD-1 has been developed by inserting, within the mouse PD-1 locus, a chimeric PD-1 with a human extracellular domain, a murine transmembrane domain and a murine intracellular domain. hVISTA has been developed by inserting, within the mouse VISTA locus, a chimeric VISTA with a human extracellular, human transmembrane and murine intracellular domain.

Both hPD-1 and hVISTA expressions are regulated by endogenous mouse promoters.

Applications in immuno-oncology

The hPD-1/hVISTA mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint PD-1 and/or VISTA in fully immunocompetent mice.

Humanized tumor cell line MC38-hPD-L1:
Use our hPD-1/hVISTA mouse model together with our cell line MC38-hPD-L1 as a syngeneic tumor model for testing the efficacy of combination therapies targeting human PD-1 and human PD-L1.

hPD-1/hVISTA features

• The PD-1 and VISTA extracellular domains are entirely humanized
• Physiological regulation and expression pattern of the human PD-1 and VISTA
• Preservation of the target-ligand interaction
• Fully functional mouse immune system
• Lack of expression of the murine target gene, thus avoiding cross-reactivity

hPD-1/hVISTA validation*

* For more validation data please consult our hPD-1 and hVISTA webpages, or  contact us.

Ready to be shipped to your lab

• Cohorts available upon request
• Studies can be carried out at your site or at your favorite CRO
• SOPF certification and worldwide delivery by professional breeders
• Models provided with FTO on patent-protected technologies used for model generation