Preclinical Double-Humanized PD-1/VISTA Mouse Model

Humanized Immune Checkpoint Mouse Models

Design of the hPD‑1/hVISTA mouse

The double-humanized PD-1 and VISTA mouse model (hPD-1/hVISTA) was generated by intercrossing hPD-1 and hVISTA mice.

hPD-1 has been developed by inserting, within the mouse PD-1 locus, a chimeric PD-1 with a human extracellular domain, a murine transmembrane domain and a murine intracellular domain. hVISTA has been developed by inserting, within the mouse VISTA locus, a chimeric VISTA with a human extracellular, human transmembrane and murine intracellular domain.

Both hPD-1 and hVISTA expressions are regulated by endogenous mouse promoters.

Applications in immuno-oncology

The hPD-1/hVISTA mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint PD-1 and/or VISTA in fully immunocompetent mice.

Humanized tumor cell line MC38-hPD-L1:
Use our hPD-1/hVISTA mouse model together with our cell line MC38-hPD-L1 as a syngeneic tumor model for testing the efficacy of combination therapies targeting human PD-1 and human PD-L1.

 

Request a quote here to get pricing, offers and hPD-1/hVISTA model information by phone or email.

 

hPD-1/hVISTA features

  • The PD-1 and VISTA extracellular domains are entirely humanized
  • Physiological regulation and expression pattern of the human PD-1 and VISTA
  • Preservation of the target-ligand interaction
  • Fully functional mouse immune system
  • Lack of expression of the murine target gene, thus avoiding cross-reactivity

hPD-1/hVISTA validation*

hPD-1+hVISTA ICP model validation 1

hPD-1 expression pattern in hPD-1/hVISTA mice recapitulates mPD-1 in wild-type mice

hPD-1 and mPD-1 expression on αCD3/αCD28-activated splenocytes on Tregs (A) and conventional CD4+ (B) at day 2 of culture, and on CD8+ T cells (C) at the indicated time points.

hPD-1+hVISTA ICP model validation 2

hVISTA expression pattern in hPD-1/hVISTA mice recapitulates mVISTA in wild-type mice

hVISTA and mVISTA expression on freshly isolated splenocytes on Tregs, conventional CD4+, and CD8+ T cells.

* For more validation data please consult our hPD-1 and hVISTA webpages, or  contact us.

Ready to be shipped to your lab

  • Cohorts available upon request
  • Studies can be carried out at your site or at your favorite CRO
  • VAF Elite/SOPF certification and worldwide delivery by professional breeders
  • Models provided with FTO on patent-protected technologies used for model generation


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Follow this link humanized immune checkpoint (ICP) mouse models, or contact us.