Preclinical Humanized GITR Mouse Model

Humanized Immune Checkpoint Mouse Models

Design of the hGITR mouse

The humanized GITR mouse model (hGITR) is developed by Knockin at the mouse GITR locus and driven by the endogenous promoter. It expresses a chimeric GITR with a human extracellular and murin intracellular domain. The human isoforms display diversity within the intracellular domain, and are expressed in case they support different signaling functions.

Applications in immuno-oncology

The hGITR mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint GITR in fully immunocompetent mice.

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hGITR features

The GITR extracellular domain is entirely humanized
Physiological regulation and expression pattern of the human GITR
Fully functional mouse immune system
Lack of expression of the murine target gene, thus avoiding cross-reactivity

See also:

Triple-humanized hPD1/hGITR/hGITRL mouse model
Double-humanized hGITR/hGITRL mouse model
Reporter hGITR/Foxp3 mouse model

hGITR validation*

hGITR ICP model validation 1

hGITR expression is induced after stimulation

Expression of human GITR on aCD3/aCD28-stimulated splenocytes (2 days), analyzed by flow cytometry on Tregs, conventional CD4 (CD4conv), CD8 and B cells.

hGITR ICP model validation 2

In vivo anti-tumor effect in response to anti-hGITR mAb treatment

hGITR homozygous mice with MB49 bladder cancer at day 0 were treated with vehicle or ahGITR mAb on days 7, 10 and 13. Tumor growth was followed until day 30. Unpaired t-test statistics: * p<0.05, ** p<0.005.

* For more validation data please contact us.

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Studies can be carried out at your site or at your favorite CRO

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Models provided with FTO on patent-protected technologies used for model generation

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