genO-BRGSF-HIS mice: A humanized mouse model for assessment of Treg-targeting therapies

Background: Therapies targeting regulatory T cells (Tregs) have emerged as a promising strategy to overcome immune suppression and enhance anti-tumor immunity. Tregs maintain immune tolerance under physiological conditions, but within the tumor microenvironment they promote immune evasion and resistance to immunotherapy. Selective depletion or functional modulation of Tregs can restore effector T-cell activity and improve responses to checkpoint inhibitors and other immunotherapies. However, the development of Treg-targeting approaches requires preclinical models that accurately recapitulate human Treg biology, including phenotype, activation status, and distribution in peripheral tissues and tumors. Conventional mouse models often fail to reflect these human-specific features, limiting their predictive value for safety and efficacy. Humanized models that support the development and long-term engraftment of functional Tregs and mimic human immune–tumor interactions are therefore essential to bridge the translational gap and guide clinical development of next-generation immunotherapies.

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