Humanized MC38-hPD-L1-hHER2-LZ Reporter Tumor Cell Line

Reporter Immune Checkpoint Cell Lines

Design of the MC38-hPD‑L1-hHER2-LZ cell line

The MC38 cell line was derived from C57BL/6 murine colon adenocarcinoma cells. They form solid tumors upon subcutaneous injection in immunocompetent mice.

We generated a MC38-hPD-L1-hHER2-LZ clonal cell line expressing high levels of human PD-L1 (CD274 NCBI GeneID: 29126) and human HER2 (ERBB2 NCBI GeneID: 2064), and an optimized dual fusion reporter luciferase-ZsGreen (LZ). These cells are invalidated for murine Pd-l1 (Cd274, NCBI Gene ID: 60533). MC38-hPD-L1-hHER2-LZ cells form solid tumors in vivo. Isogenic wild-type (WT) and luciferase-ZsGreen (LZ) control clones are available.



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MC38-hPD-L1-hHER2-LZ features

  • Express high levels of full human PD-L1 and HER2
  • Express a luciferase-ZsGreen (LZ) fusion reporter
  • Form solid tumors upon subcutaneous injection in fully immunocompetent mice
  • Injected cells can be followed in vivo and ex vivo with our LZ reporter


MC38-hPD-L1-hHER2-LZ validation*

MC38-hPD-L1-hHER2-LZ_img01MC38-hPD-L1-hHER2-LZ cells homogeneously express human PD‑L1 and HER2 in vitro

Expression analysis by flow cytometry. Human PD-L1 and HER2 expression assessed in MC38 parental cell line (top left), human control cell line – RKO (bottom left) and BT474 (bottom right), and MC38-hPD-L1-hHER2-LZ cell line (top right). Human HER2 was detected using Trastuzumab antibody.

MC38-hPD-L1-hHER2-LZ_img02MC38-hPD-L1-hHER2-LZ cells form tumors in vivo

C57/Bl6 mice were injected (s.c.) with 2x106 MC38-hPD-L1-LZ cells (left) or MC38-hPD-L1-hHER2-LZ cells (right). Tumor growth was measured every 2 to 3 days. Tumor size (mm3) = (width²*length)/2.

* For more validation data please contact us.

Ready to be shipped to your lab

  • Biosafety level BSL1, according to Kerafast guidelines (parental cell line provider)
  • Studies can be carried out at your site or at your favorite CRO
  • Models provided with FTO on patent-protected technologies used for model generation

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