Design of the MC38-hPD‑L1-hCD47-LZ cell line

The MC38 cell line was derived from C57BL/6 murine colon adenocarcinoma cells. They form solid tumors upon subcutaneous injection in immunocompetent mice.

We generated a MC38-hPD-L1-hCD47-LZ clonal cell line expressing high levels of human PD-L1 (CD274 NCBI GeneID: 29126) and human CD47 (NCBI GeneID: 961), and an optimized dual fusion reporter luciferase-ZsGreen (LZ). These cells are invalidated for murine Pd-l1 (Cd274, NCBI Gene ID: 60533). MC38-hPD-L1-hCD47-LZ cells form solid tumors in vivo, most efficiently in hCD47/hSIRPα mice. Isogenic wild-type (WT) and luciferase-ZsGreen (LZ) control clones are available.

MC38-hPD-L1-hCD47-LZ features

  • Form solid tumors upon subcutaneous injection in fully immunocompetent mice
  • PD-L1 and CD47 are entirely humanized
  • Express a luciferase-ZsGreen (LZ) fusion reporter
  • Injected cells can be followed in vivo and ex vivo with our LZ reporter

Validation

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