Preclinical humanized genO‑hTFRC mouse model

The fully immunocompetent genO‑hTFRC mouse model enables in vivo efficacy assessment and profiling of compounds targeting human TFRC (CD71, TfR1) and aiming to deliver therapeutic agents through the blood brain barrier.

Applications & Features
Model Delivery & Logistics
Resources
Validation Data

Applications of the genO‑hTFRC mouse model

Useful applications for researchers working on different biomedical fields, such as neuroscience, immuno-oncology and inflammation:

Improve the delivery of therapeutic agents aiming to cross the blood-brain barrier (BBB), via human transferrin receptor (TFRC) binding and test their efficacy
Assess the efficacy of immunotherapies in cancers that overexpress TFRC (CD71, TfR1)
Evaluate the in vivo PK profile and toxicity of TFRC-binding therapeutics

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Features

Physiological regulation and expression pattern of human TFRC
Lack of expression of the murine target gene
Fully functional mouse immune system
Available on C57BL/6N genetic background

Clients

Lundbeck

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Inatherys

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Model Delivery & Care

Read our FAQ on delivery & care here
Orders are made directly with genOway; breeding, housing and delivery are outsourced
Breeding facilities in the US and Europe through our trusted partners:

Quick, compliant, and reliable door-to-door delivery worldwide

Models with certified SOPF health status from AAALAC-accredited and PHS-assured professional breeders

Validation data

Human TFRC expression mirrors expression of mouse TFRC on different cell types
Expression of TFRC (anti-mouse TFRC clone C2, anti-human TFRC clone OKT-9) on TER-119⁺ cells from freshly isolated bone marrow cells (A), blood (B), and splenocytes (C), gated on single viable cells.
Human transferrin internalization is efficient in cells from genO‑hTFRC mice
Splenocytes of genO‑hTFRC mice were incubated with human conjugate pHrodo red transferrin for 2 hours at 37°C. Internalization of human transferrin (Tf) was confirmed by the increase of the MFI values of pHrodo Tf.
Specific activity of hTFRC: pre-incubation of splenocytes with pHrodo competitor, rh-transferrin, reduced the level of pHrodo internalization.
Targeting human TFRC improves shuttling to the brain of the anti-BACE1 antibody
Combination of anti-hTFRC/anti-BACE1, but not anti-BACE1 alone, enables the biodistribution of IgG in the brain and the reduction of amyloid-β.
Rational: anti-BACE1 inhibits amyloid-b(Ab) production in vivo (Atwal et al.,2011)
genO‑hTFRC mice were treated with different anti-BACE1/anti-TFRC antibodies for 24h. Concentrations of hIgG and Ab were quantified by ELISA-based assay in plasma and brain homogenates.
Average weight of genO-hTFRC mice (males and females)

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Preclinical humanized genO‑hTFRC mouse model

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Preclinical humanized genO‑hTFRC mouse model

Applications of the genO‑hTFRC mouse model

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