Conventional Knockin Mouse Models

For academic research: Tissue-specific gene expression Cell lineage tracking & sorting Cell fate mapping

For bio-pharmaceutical research & development: Tissue-specific gene expression Cell lineage tracking & sorting Cell fate mapping

+	Preservation of the physiological gene expression

-	Level of expression might be too weak → Can be compensated by the use of a WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element)

Torben Johann Hausrat, Philipp C Janiesch, Petra Breiden, David Lutz, Sabine Hoffmeister-Ullerich, Irm Hermans-Borgmeyer, Antonio Virgilio Failla, Matthias Kneussel.
Disruption of tubulin-alpha4a polyglutamylation prevents aggregation of hyper-phosphorylated tau and microglia activation in mice
Nat Commun. 2022 Jul 20

Barbara Ben Yamin, Sana Ahmed-Seghir, Junya Tomida, Emmanuelle Despras, Caroline Pouvelle, Andrey Yurchenko, Jordane Goulas, Raphael Corre, Quentin Delacour, Nathalie Droin, Philippe Dessen, Didier Goidin, Sabine S Lange, Sarita Bhetawal, Maria Teresa Mitjavila-Garcia, Giuseppe Baldacci, Sergey Nikolaev, Jean Charles Cadoret, Richard D Wood, Patricia L Kannouche.
DNA polymerase zeta contributes to heterochromatin replication to prevent genome instability.
EMBO J. 2021 Sep 17

Qinchuan Wang, Erick O Hernández-Ochoa, Meera C Viswanathan, Ian D Blum, Danh C Do, Jonathan M Granger, Kevin R Murphy, An-Chi Wei, Susan Aja, Naili Liu, Corina M Antonescu, Liliana D Florea, C Conover Talbot Jr, David Mohr, Kathryn R Wagner, Sergi Regot, Richard M Lovering, Peisong Gao, Mario A Bianchet, Mark N Wu, Anthony Cammarato, Martin F Schneider, Gabriel S Bever, Mark E Anderson.
CaMKII oxidation is a critical performance/disease trade-off acquired at the dawn of vertebrate evolution.
Nat Commun. 2021 May 26

Jamie O Brett, Marina Arjona, Mika Ikeda, Marco Quarta, Antoine de Morrée, Ingrid M Egner, Luiz A Perandini, Heather D Ishak, Armon Goshayeshi, Daniel I Benjamin, Pieter Both, Cristina Rodríguez-Mateo, Michael J Betley, Tony Wyss-Coray, Thomas A Rando.
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1.
Nat Metab. 2020 Apr.

Hesham Soliman, Ben Paylor, R Wilder Scott, Dario R Lemos, ChihKai Chang, Martin Arostegui, Marcela Low, Christina Lee, Daniela Fiore, Paola Braghetta, Vendula Pospichalova, Christina E Barkauskas, Vladimir Korinek, Alessandra Rampazzo, Kathleen MacLeod, T Michael Underhill, Fabio M V Rossi.
Pathogenic Potential of Hic1-Expressing Cardiac Stromal Progenitors.
Cell Stem Cell. 2020 Feb 6.

Scott RW, Arostegui M, Schweitzer R, Rossi FMV, Underhill TM.
Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration.
Cell Stem Cell. 2019 Dec 5.

Sen NE, Canet-Pons J, Halbach MV, Arsovic A, Pilatus U, Chae WH, Kaya ZE, Seidel K, Rollmann E, Mittelbronn M, Meierhofer D, De Zeeuw CI, Bosman LWJ, Gispert S, Auburger G.
Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation.
Neurobiol Dis. 2019 Jul 31.

Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, Moss SJ.
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Cell Rep. 2019 Jul 16.

Leschik J, Eckenstaler R, Endres T, Munsch T, Edelmann E, Richter K, Kobler O, Fischer KD, Zuschratter W, Brigadski T, Lutz B, Lessmann V.
Prominent Postsynaptic and Dendritic Exocytosis of Endogenous BDNF Vesicles in BDNF-GFP Knock-in Mice.
Mol Neurobiol. 2019 Mar 30.

Hines RM, Maric HM, Hines DJ, Modgil A, Panzanelli P, Nakamura Y, Nathanson AJ, Cross A, Deeb T, Brandon NJ, Davies P, Fritschy JM, Schindelin H, Moss SJ.
Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin.
Nat Commun. 2018 Aug 7.

Thorens B, Tarussio D, Maestro MA, Rovira M, Heikkilä E, Ferrer J
Ins1 Cre knock-in mice for beta cell-specific gene recombination.
Diabetologia. 2014 Dec 11.

Dodd GT, Worth AA, Nunn N, Korpal AK, Bechtold DA, Allison MB, Myers MG Jr, Statnick MA, Luckman SM
The Thermogenic Effect of Leptin Is Dependent on a Distinct Population of Prolactin-Releasing Peptide Neurons in the Dorsomedial Hypothalamus.
Cell Metab. 2014 Aug 27.

Nicoleta Moisoi, Valentina Fedele, Jennifer Edwards, L Miguel Martins.
Loss of PINK1 enhances neurodegeneration in a mouse model of Parkinson's disease triggered by mitochondrial stress.
Neuropharmacology. 2014 Feb.

Sarzi E, Angebault C, Seveno M, Gueguen N, Chaix B, Bielicki G, Boddaert N, Mausset-Bonnefont AL, Cazevieille C, Rigau V, Renou JP, Wang J, Delettre C, Brabet P, Puel JL, Hamel CP, Reynier P, Lenaers G.
The human OPA1delTTAG mutation induces premature age-related systemic neurodegeneration in mouse.
Brain. 2012 Dec.

Damrath E, Heck MV, Gispert S, Azizov M, Nowock J, Seifried C, Rüb U, Walter M, Auburger G.
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
PLoS Genet. 2012 Aug 30.

Husse J, Zhou X, Shostak A, Oster H, Eichele G.
Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN.
J Biol Rhythms. 2011 Oct.

Sven Müller-Loennies 1, Giovanna Galliciotti, Katrin Kollmann, Markus Glatzel, Thomas Braulke.
A novel single-chain antibody fragment for detection of mannose 6-phosphate-containing proteins: application in mucolipidosis type II patients and mice.
Am J Pathol. 2010 Jul.