Conventional Knockin Mouse Models

 



Get supplemental information, a quote, and estimated timeframe to generate your Knockin mouse.

 

A conventional or constitutive Knockin mouse defines a model in which an exogenous gene is inserted in a mouse gene locus of interest.

The inserted gene or gene sequence expression is regulated as the mouse gene was, which provides a physiologically relevant model.

 

Infographic: Conventional Knockin mouse model

Typical applications for conventional Knockin mouse models


For academic research:
  • Tissue-specific gene expression
  • Cell lineage tracking & sorting
  • Cell fate mapping
For bio-pharmaceutical research & development:
  • Tissue-specific gene expression
  • Cell lineage tracking & sorting
  • Cell fate mapping

Strengths and limitations of conventional Knockin mouse models

+
  • Preservation of the physiological gene expression
-
  • Level of expression might be too weak
    → Can be compensated by the use of a WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element)


Publications on our conventional Knockin mouse models

Publications

Thorens B, Tarussio D, Maestro MA, Rovira M, Heikkilä E, Ferrer J
Ins1 Cre knock-in mice for beta cell-specific gene recombination.
Diabetologia. 2014 Dec 11.

Dodd GT, Worth AA, Nunn N, Korpal AK, Bechtold DA, Allison MB, Myers MG Jr, Statnick MA, Luckman SM
The Thermogenic Effect of Leptin Is Dependent on a Distinct Population of Prolactin-Releasing Peptide Neurons in the Dorsomedial Hypothalamus.
Cell Metab. 2014 Aug 27.

Sarzi E, Angebault C, Seveno M, Gueguen N, Chaix B, Bielicki G, Boddaert N, Mausset-Bonnefont AL, Cazevieille C, Rigau V, Renou JP, Wang J, Delettre C, Brabet P, Puel JL, Hamel CP, Reynier P, Lenaers G.
The human OPA1delTTAG mutation induces premature age-related systemic neurodegeneration in mouse.
Brain. 2012 Dec.

Damrath E, Heck MV, Gispert S, Azizov M, Nowock J, Seifried C, Rüb U, Walter M, Auburger G.
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
PLoS Genet. 2012 Aug 30.

Husse J, Zhou X, Shostak A, Oster H, Eichele G.
Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN.
J Biol Rhythms. 2011 Oct.

Follow these links if you are looking for another Knockin mouse model or customized mouse model.