Conventional Knockin Mouse Models

 

Get supplemental information, a quote, and estimated timeframe to generate your Knockin mouse.

 

A conventional or constitutive Knockin mouse defines a model in which an exogenous gene is inserted in a mouse gene locus of interest.

The inserted gene or gene sequence expression is regulated as the mouse gene was, which provides a physiologically relevant model.

 

Infographic: Conventional Knockin mouse model

Typical applications for conventional Knockin mouse models


For academic research:
  • Tissue-specific gene expression
  • Cell lineage tracking & sorting
  • Cell fate mapping
For bio-pharmaceutical research & development:
  • Tissue-specific gene expression
  • Cell lineage tracking & sorting
  • Cell fate mapping

Strengths and limitations of conventional Knockin mouse models

+
  • Preservation of the physiological gene expression
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  • Level of expression might be too weak
    → Can be compensated by the use of a WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element)


Publications on our conventional Knockin mouse models

Publications

Jamie O Brett, Marina Arjona, Mika Ikeda, Marco Quarta, Antoine de Morrée, Ingrid M Egner, Luiz A Perandini, Heather D Ishak, Armon Goshayeshi, Daniel I Benjamin, Pieter Both, Cristina Rodríguez-Mateo, Michael J Betley, Tony Wyss-Coray, Thomas A Rando.
Exercise rejuvenates quiescent skeletal muscle stem cells in old mice through restoration of Cyclin D1.
Nat Metab. 2020 Apr.

Hesham Soliman, Ben Paylor, R Wilder Scott, Dario R Lemos, ChihKai Chang, Martin Arostegui, Marcela Low, Christina Lee, Daniela Fiore, Paola Braghetta, Vendula Pospichalova, Christina E Barkauskas, Vladimir Korinek, Alessandra Rampazzo, Kathleen MacLeod, T Michael Underhill, Fabio M V Rossi.
Pathogenic Potential of Hic1-Expressing Cardiac Stromal Progenitors.
Cell Stem Cell. 2020 Feb 6.

Scott RW, Arostegui M, Schweitzer R, Rossi FMV, Underhill TM.
Hic1 Defines Quiescent Mesenchymal Progenitor Subpopulations with Distinct Functions and Fates in Skeletal Muscle Regeneration.
Cell Stem Cell. 2019 Dec 5.

Sen NE, Canet-Pons J, Halbach MV, Arsovic A, Pilatus U, Chae WH, Kaya ZE, Seidel K, Rollmann E, Mittelbronn M, Meierhofer D, De Zeeuw CI, Bosman LWJ, Gispert S, Auburger G.
Generation of an Atxn2-CAG100 knock-in mouse reveals N-acetylaspartate production deficit due to early Nat8l dysregulation.
Neurobiol Dis. 2019 Jul 31.

Nathanson AJ, Zhang Y, Smalley JL, Ollerhead TA, Rodriguez Santos MA, Andrews PM, Wobst HJ, Moore YE, Brandon NJ, Hines RM, Davies PA, Moss SJ.
Identification of a Core Amino Acid Motif within the α Subunit of GABAARs that Promotes Inhibitory Synaptogenesis and Resilience to Seizures.
Cell Rep. 2019 Jul 16.

Leschik J, Eckenstaler R, Endres T, Munsch T, Edelmann E, Richter K, Kobler O, Fischer KD, Zuschratter W, Brigadski T, Lutz B, Lessmann V.
Prominent Postsynaptic and Dendritic Exocytosis of Endogenous BDNF Vesicles in BDNF-GFP Knock-in Mice.
Mol Neurobiol. 2019 Mar 30.

Hines RM, Maric HM, Hines DJ, Modgil A, Panzanelli P, Nakamura Y, Nathanson AJ, Cross A, Deeb T, Brandon NJ, Davies P, Fritschy JM, Schindelin H, Moss SJ.
Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin.
Nat Commun. 2018 Aug 7.

Thorens B, Tarussio D, Maestro MA, Rovira M, Heikkilä E, Ferrer J
Ins1 Cre knock-in mice for beta cell-specific gene recombination.
Diabetologia. 2014 Dec 11.

Dodd GT, Worth AA, Nunn N, Korpal AK, Bechtold DA, Allison MB, Myers MG Jr, Statnick MA, Luckman SM
The Thermogenic Effect of Leptin Is Dependent on a Distinct Population of Prolactin-Releasing Peptide Neurons in the Dorsomedial Hypothalamus.
Cell Metab. 2014 Aug 27.

Nicoleta Moisoi, Valentina Fedele, Jennifer Edwards, L Miguel Martins.
Loss of PINK1 enhances neurodegeneration in a mouse model of Parkinson's disease triggered by mitochondrial stress.
Neuropharmacology. 2014 Feb.

Sarzi E, Angebault C, Seveno M, Gueguen N, Chaix B, Bielicki G, Boddaert N, Mausset-Bonnefont AL, Cazevieille C, Rigau V, Renou JP, Wang J, Delettre C, Brabet P, Puel JL, Hamel CP, Reynier P, Lenaers G.
The human OPA1delTTAG mutation induces premature age-related systemic neurodegeneration in mouse.
Brain. 2012 Dec.

Damrath E, Heck MV, Gispert S, Azizov M, Nowock J, Seifried C, Rüb U, Walter M, Auburger G.
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
PLoS Genet. 2012 Aug 30.

Husse J, Zhou X, Shostak A, Oster H, Eichele G.
Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN.
J Biol Rhythms. 2011 Oct.

Sven Müller-Loennies 1, Giovanna Galliciotti, Katrin Kollmann, Markus Glatzel, Thomas Braulke.
A novel single-chain antibody fragment for detection of mannose 6-phosphate-containing proteins: application in mucolipidosis type II patients and mice.
Am J Pathol. 2010 Jul.

Follow these links if you are looking for another Knockin mouse model or customized mouse model.