Conventional Knockin Mouse Models
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A conventional or constitutive Knockin mouse defines a model in which an exogenous gene is inserted in a mouse gene locus of interest.
The inserted gene or gene sequence expression is regulated as the mouse gene was, which provides a physiologically relevant model.
Typical applications for conventional Knockin mouse models
For academic research:
For bio-pharmaceutical research & development:
Strengths and limitations of conventional Knockin mouse models
- Preservation of the physiological gene expression
- Level of expression might be too weak
→ Can be compensated by the use of a WPRE (Woodchuck Hepatitis Virus Post-transcriptional Regulatory Element)
Publications on our conventional Knockin mouse models
Leschik J, Eckenstaler R, Endres T, Munsch T, Edelmann E, Richter K, Kobler O, Fischer KD, Zuschratter W, Brigadski T, Lutz B, Lessmann V.
Prominent Postsynaptic and Dendritic Exocytosis of Endogenous BDNF Vesicles in BDNF-GFP Knock-in Mice.
Mol Neurobiol. 2019 Mar 30.
Hines RM, Maric HM, Hines DJ, Modgil A, Panzanelli P, Nakamura Y, Nathanson AJ, Cross A, Deeb T, Brandon NJ, Davies P, Fritschy JM, Schindelin H, Moss SJ.
Developmental seizures and mortality result from reducing GABAA receptor α2-subunit interaction with collybistin.
Nat Commun. 2018 Aug 7.
Thorens B, Tarussio D, Maestro MA, Rovira M, Heikkilä E, Ferrer J
Ins1 Cre knock-in mice for beta cell-specific gene recombination.
Diabetologia. 2014 Dec 11.
Dodd GT, Worth AA, Nunn N, Korpal AK, Bechtold DA, Allison MB, Myers MG Jr, Statnick MA, Luckman SM
The Thermogenic Effect of Leptin Is Dependent on a Distinct Population of Prolactin-Releasing Peptide Neurons in the Dorsomedial Hypothalamus.
Cell Metab. 2014 Aug 27.
Sarzi E, Angebault C, Seveno M, Gueguen N, Chaix B, Bielicki G, Boddaert N, Mausset-Bonnefont AL, Cazevieille C, Rigau V, Renou JP, Wang J, Delettre C, Brabet P, Puel JL, Hamel CP, Reynier P, Lenaers G.
The human OPA1delTTAG mutation induces premature age-related systemic neurodegeneration in mouse.
Brain. 2012 Dec.
Damrath E, Heck MV, Gispert S, Azizov M, Nowock J, Seifried C, Rüb U, Walter M, Auburger G.
ATXN2-CAG42 sequesters PABPC1 into insolubility and induces FBXW8 in cerebellum of old ataxic knock-in mice.
PLoS Genet. 2012 Aug 30.
Husse J, Zhou X, Shostak A, Oster H, Eichele G.
Synaptotagmin10-Cre, a driver to disrupt clock genes in the SCN.
J Biol Rhythms. 2011 Oct.
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