Customized Knockin Mouse Models

A Knockin mouse defines a model in which a gene sequence is altered via one-for-one substitution or by addition of DNA sequence information.

This technology is often used for the creation of models to study, for example:

  • Transgene overexpression
  • Human disease
  • Compound efficacy
  • Promoter activity
  • Inducible gene expression
  • Target protein expression, localization, and trafficking
  • Mutant protein expression
  • Cell trafficking

Regardless of the desired model you wish to develop, we provide free consulting services, for:

  • A thorough assessment of the risks and benefits
  • An analysis of the potential trouble spots during development
  • Choosing the best approach to create the physiologically most relevant model
  • An accurate timeline of the entire model development process

Contact our scientific consultant, and get guidance and precise information on your specific Knockin design.

Below you will find our different types of Knockin designs:

Humanized mice - Replaces a mouse gene with its human counterpart.

Point mutation mice - Introduces a specific point mutation at a specific nucleotide position within the gene of interest.

Inducible point mutation mice - Introduces a point mutation in a time-controlled manner.

Reporter mice - Introduces a reporter gene into the locus of a target gene in order to monitor the expression of that gene at the transcriptional or translational level. May also be used to monitor protein trafficking.

Rosa26 and Hprt targeted transgenesis - Introduces a safe and targeted insertion of a transgene into the Rosa26 or Hprt locus.