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Humanized ICP mouse and cell lines
Humanized PD-1/PD-L1 mouse and cell line
Humanized PD-1 & GITR mouse
T-cell engager
Assess toxicity with ICP
Immuno-oncolology models
The double-humanized CTLA-4 and LAG3 mouse model (hCTLA-4/hLAG3) was generated by intercrossing hCTLA-4 and hLAG3 mice.
The design of hCTLA-4, developed by Knockin at the mouse Ctla-4 locus, enables the expression of: a) the full-length chimeric CTLA-4 isoform (human extracellular domain and mouse intracellular domain); and b) the soluble isoform (human extracellular domain).
The hLAG3 humanized model, developed by Knockin at the mouse Lag3 locus, expresses the full length chimeric LAG3 isoform (human signal peptide, human extracellular domains, and mouse transmembrane and intracellular domains).
Both hCTLA-4 and hLAG3 expressions are regulated by endogenous mouse promoters.
Splenocytes from hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C,D). hCTLA-4 and mCTLA-4 expressions were evaluated on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.
Splenocytes from wild-type or hCTLA-4/hLAG3 double-homozygous mice were activated with coated αCD3/αCD28 and mIL-2 for 2 days (A, B) or indicated time (C, D). hLAG3 and mLAG3 expression on A) Tregs (viable, CD3+CD4+CD25+Foxp3+), B) conventional CD4+ (viable, CD3+CD4+Foxp3-), and C, D) CD8+ (viable, CD3+CD8+) T cells.
Humanized ICP mouse and cell lines
Humanized PD-1/PD-L1 mouse and cell line
Humanized PD-1 & GITR mouse
T-cell engager
Assess toxicity with ICP
Immuno-oncolology models
The hGITR/Foxp3 mouse enables the in vivo efficacy assessment and profiling of immuno-oncology agents targeting the human immune checkpoint GITR. The red fluorescent protein regulated under the Foxp3 promoter allows you to efficiently monitor and sort Foxp3-expressing cells from different lymphocyte lineages and lymphoid organs.
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