IL-4 is the canonical marker of Th2 cells and is known to induce differentiation of naive helper T cells to Th2 cells by polarizing the immune response toward a humoral effector response.

In contrary to other existing IL-4 reporter models generated by random insertion, this Knockin mouse line preserves the physiological expression of IL-4 and enables monitoring of Th2 activity.

Features

No deregulation of endogenous IL-4 expression:

  • Endogenous IL-4 is expressed and functional: reporter gene inserted in the 3'UTR of the endogenous IL-4 locus
  • Genetic manipulation inserted is controlled
  • Reporter faithfully mirrors IL-4 expression

Suitable model for your Th2 polarization monitoring studies:

  • IL-4 expressing cells could be directly detected by monitoring eGFP expression (Fig. 1)
  • Uncompromised immune system: intact production of IL-4 in response to infection or challenge (Fig. 2)
  • Optimum monitoring of Th2 cell trafficking and cell sorting

Validation

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