Design of the hPD-1/hPD-L1 mouse

The double-humanized PD‑1 and PD-L1 (hPD-1/hPD-L1) mouse model was generated by intercrossing hPD-1 and hPD-L1 mice.

The PD-1 humanized model, developed by Knockin at the mouse Pd-1 locus, expresses a chimeric PD-1: human extracellular and mouse transmembrane and intracellular domains.

The hPD-L1 humanized model, developed by Knockin at the mouse Pd-L1 locus, expresses a chimeric PD-L1 (human extracellular domains and murine transmembrane and intracellular domains).

Both hPD-1 and hPD-L1 expressions are regulated by endogenous mouse promoters.

hPD-1/hPD-L1 features

  • PD-1 and PD-L1 extracellular domains are entirely humanized
  • hPD-1 and hPD-L1 expression driven by the respective endogenous mouse promoter
  • Preservation of the target-ligand interaction
  • Fully functional mouse immune system
  • Lack of expression of the murine target gene, thus avoiding cross-reactivity

Validation

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