Immune Checkpoint Catalog Cell Lines
Humanized and Knockout immune checkpoint cell lines can be used for in vivo tumor growth assays in syngeneic models and in vitro mechanistic studies.
- Biosafety level BSL1, according to parental cell line provider guidelines
- Studies can be carried out at your site or at your favorite CRO
- Models provided with FTO on patent-protected technologies used for model generation
We have developed humanized and knockout murine tumor cell lines to be utilized in the two major genetic backgrounds used for syngeneic models: C57BL6 and Balb/c.
Each genetic background shows specificities that can be of interest, depending on the target and compound studied. For example, it has been shown that genetic background influences the immune response, cellular and humoral, to vaccines (Zeng et al., ClinExpImmunol, 2016).
Humanized tumor cell lines for syngeneic models
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Target: PD‑L1 The MC38-hPD-L1 clonal tumor cell line expresses high levels of human PD-L1, and forms solid tumors in vivo. These cells only express the human PD-L1 as they are invalidated for murine Pd‑l1. |
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Target: PD‑L1 The CT26-hPD-L1 clonal tumor cell line expresses human PD-L1. Learn more |
Knockout tumor cell lines
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Target: Pd‑l1 The MC38-Pd-l1 Knockout clonal tumor cell line is invalidated for murine Pd-l1 and can be used for target validation and mechanistic studies. |
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Target: Pd‑l1 CT26-Pd-l1 Knockout cells are invalidated for murine Pd-l1. |
Humanized tumor cell lines with reporter
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Target: PD‑L1 The MC38-hPD-L1-LZ clonal tumor cell line expresses high levels of human PD-L1 and luciferase-ZsGreen (LZ) fusion protein for in vivo imaging and ex vivo tracking. |
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Targets: PD‑L1 & CD47 The MC38-hPD-L1-hCD47-LZ clonal tumor cell line expresses high levels of human PD-L1, human CD47 and luciferase-ZsGreen (LZ) fusion protein for in vivo imaging and ex vivo tracking. |
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Targets: PD‑L1 & HER2 The MC38-hPD-L1-hCDHER2-LZ clonal tumor cell line expresses high levels of human PD-L1, human HER2 (ERBB2) and luciferase-ZsGreen (LZ) fusion protein for in vivo imaging and ex vivo tracking. |
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Target: CD39 The MC38-hCD39-LZ cells homogeneously express human CD39 in vitro, and form infiltrated tumors in immunocompetent mice. |
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Target: CD39 The CT26-hCD39-LZ clonal tumor cell line expresses high levels of human/mouse chimeric CD39 and luciferase-ZsGreen (LZ) fusion protein for in vivo imaging and ex vivo tracking. |
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Target: EpCAM The CT26-hEpCAM-LZ clonal cell line expresses high levels of human EpCAM and luciferase-ZsGreen (LZ) fusion protein for in vivo imaging and ex vivo tracking. |
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Target: CD20 The EL4-hCD20-LZ clonal cell line expresses high levels of human CD20 and an optimized dual reporter luciferase-ZsGreen (LZ). |
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Target: CD19 The EL4-hCD19-LZ clonal cell line expresses high levels of human CD19 and an optimized dual reporter luciferase-ZsGreen (LZ). |
Further reading
If you are looking for another target, get in touch with us.